use prefix []or [-]not [+]and [=]has feature [!]exclude feature ie. 'interleukin-6 -animal +phenotypic =protein !tumor'

Displaying 10 papers, 165 pages, start at 1, 7 Hits
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ESVE

Xenotransfusion of canine blood to cats may be a life-saving procedure when treating an acute anaemic syndrome and compatible feline blood cannot be obtained. Published evidence in a limited number of cases dating from the 1960s indicates that cats do not appear to have naturally-occurring antibodies against canine red blood cell antigens. In fact compatibility tests before the first transfusion did not demonstrate evidence of agglutination or haemolysis of canine erythrocytes in feline serum and no severe acute adverse reactions have been reported in cats receiving a single transfusion with canine blood. Severe acute reactions not reported so far cannot however be excluded and we decided to perform a pilot study to evaluate the presence of naturally occurring antibodies against canine red blood cell antigens in cats and viceversa. Surplus material from diagnostic samples (blood EDTA and blood serum samples) of 13 cats and 24 dogs was used to perform test-tube major and minor cross-match tests (at 37°C, 4°C and room temperature (RT)) and blood typing, after obtaining the informed consent from owners. Hemolysis, macro-and micro-ag-glutination were investigated in each test tube and were considered markers of a positive matching. Blood from each cat was tested with blood from 2 to 6 different dogs for a total of 49 major and minor cross-matchings each one performed at the 3 different temperatures of incubation. Thirty-eight overall major cross-matchings proved positive at 37°C, 33 at RT and 39 at 4°C respectively. The minor cross-matching was positive in all but 2 tests performed at 37°C. No cat tested totally negative (37°C, 4°C, RT) at both major and minor cross-matching procedures performed towards any single dog. Ten cats experienced positive major and minor cross-matching at 37°C, RT and 4°C towards 1-3 different dogs. Five cats were positive in the major cross-match, at least at 37°C, towards 1-3 different different dogs. Seven cats obtained a positive major cross-match at RT and/or at 4°C towards 1-5 dogs. Only 2 cats tested completely negative at 37°C, RT and 4°C, in one out of the 4 different major cross-matchings performed. In conclusion, naturally occurring antibodies against canine red blood cell antigens appear to be frequently detected in cats as well as those against feline red blood cell antigens in dogs. Xenotrasfusion of canine blood to cats should only be performed after the selection of a compatible donor by means of at least a negative major cross-match test result.

ESVIM-O-4 DETECTION OF ANTI-ASPERGILLUS IMMUNOGLOBULIN

We used liver microsomes from cats (16 individual and pooled), dogs (pooled), and humans (pooled). These liver samples were incubated at 37°C in a water bath with MPA and UDP-glucuronic acid or UDP-glucose. UDP-glucose was studied since MPA glucoside is a minor metabolite in humans but may be a major metabolite in other species. HPLC-MS was used to determine concentrations of MPA-glucuronide (phenol and acyl) and MPA-glucoside (phenol) formed by incubation.

ESCG-P-4 ASSESSMENT OF IMAGE QUALITY PRODUCED BY

Five healthy Beagle dogs were included into this prospective study. All dogs were clinically examined, given a clinical score using the canine IBD activity index (CIBDAI) scoring system, also gastrointestinal endoscopy was performed. Mucosal biopsy specimens from duodenum were examined histologically from a board certified pathologist using the WSAVA grading. Biopsy incubation of 8-10 endoscopical mucosal biopsies in tissue culture medium with 3 H-labeled progesterone in the absence of any stimulation was performed. The mean age of the included dogs was 3.24+1.9 years, the mean weight was 17.8+1.8 kg. All Beagle dogs had a mean clinical score of 0+0. The mean WSAVA scoring was 2+1.2. After 4 hours, supernatant was harvested and radioactive progesterone metabolites formed were detected using high performance liquid chromatography plus liquid scintillation counting.
Five healthy Beagle dogs were included into this prospective study. All dogs were clinically examined, given a clinical score using the canine IBD activity index (CIBDAI) scoring system, also gastrointestinal endoscopy was performed. Mucosal biopsy specimens from duodenum were examined histologically from a board certified pathologist using the WSAVA grading. Biopsy incubation of 8-10 endoscopical mucosal biopsies in tissue culture medium with 3 H-labeled progesterone in the absence of any stimulation was performed. The mean age of the included dogs was 3.24 + 1.9 years, the mean weight was 17.8 + 1.8 kg. All Beagle dogs had a mean clinical score of 0 + 0. The mean WSAVA scoring was 2 + 1.2. After 4 hours, supernatant was harvested and radioactive progesterone metabolites formed were detected using high performance liquid chromatography plus liquid scintillation counting.
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ESVONC -European Society of Veterinary Oncology

Disclosures: No disclosures to report. Adjunctive use of nutraceuticals to treat human cancers has shown promise, but little work has been done in canine neoplasia. Specifically, the multi-modal effects of polyphenols and carotenoids have been established in human cell lines and rodent models, with limited work in canine cells. We have previously identified two natural ingredients, turmeric extract (TE) and rosemary extract (RE), which worked synergistically to reduce neoplastic cell growth, and had no cytotoxic effect on normal dermal fibroblasts. This combination had an additive or synergistic effect when used with the chemotherapeutic agents toceranib or doxorubicin hydrochloride. The purpose of this in-vitro study was to examine mechanisms of action of this cocktail. Three canine neoplastic cell lines representing a variety of tumors were used: C2 (mastocytoma), CMT-12 (mammary gland carcinoma), and D17 (osteosarcoma). Apoptosis was determined using Annexin V staining and by a commercially available caspase-3/7 cleavage assay. Cell cycle changes using propidium iodide staining, generation of reactive oxygen species using Dihydrorhodamine123, modulation of cellular efflux pumps, and cellular accumulation of curcumin were analyzed by flow cytometry. Perturbation of various cell signaling pathways was assessed after 12 and 24 hours of incubation by western blotting. Cells were treated with 6.3 μg/mL of extract individually, a combination (3.1 μg/mL of each extract), or vehicle control. Comparisions between these four treatment groups were analyzed using a one-way ANOVA followed by Tukey's post-hoc analyses. The combination treatment induced apoptosis in all cell lines, beyond the effects of TE alone, after 36 hours of incubation. Both extracts had a significant antioxidant effect (P < 0.05). CMT-12 cells were the most susceptible to treatment (40% Annexin V positive; 5-fold increase in caspase cleavage). The presence of RE significantly increased the cellular accumulation of TE as indicated by an increase in fluorescence, with the CMT-12 cell line showing the greatest accumulation. Western blotting showed an increase in the amount of activated c-jun N-terminal kinase (JNK), although the differences varied across cell lines. TE and RE interact synergistically to induce apoptosis in-vitro; mechanisms may include RE increasing cellular accumulation of TE and activation of JNK.