Weight: 96.36 Paper Topics: down-regulated gene expression encoding sarcoplasmic reticulum sr ca -atpase regulatory proteins sr ca -atpase isoform 2a serca2a phospholamban pln plays crucial role myocardial decompensation impairment intracellular ca 2+ cycling reported serca2a pln expressed peripheral blood mononuclear cells pbmc translatable myocardial setting significantly decrease dogs degenerative mitral valve disease aim study clarify effectively target genes reflect hemodynamic overload primary determinant myocardial distresshealthy laboratory beagles n 24 young dogs patent ductus arteriosus pda 8 pulmonic stenosis ps 5 enrolled study patients treated previously cardiovascular medication pda patients subclinical stage ps patients severe stenosis
Weight: 87.12 Paper Topics: myxomatous right ventricular rv dysfunction strongly independently predicts outcomes people mitral valve disease mmvd purpose prospective pilot study assess rv systolic function rvsf dogs different stages mmvd hypothesized rvsf differ stages disease decrease decompensated mmvdclient-owned dogs mmvd previously receiving cardiovascular medications moderate-or-severe pulmonary hypertension eligible enrollment dogs presenting acute congestive heart failure received single dose furosemide prior echocardiography validated indices rvsf measured: tricuspid annular plane systolic excursion tapse fractional area change lateral tricuspid annular longitudinal peak systolic velocity s' longitudinal global strain strain strain rate rv free wall groups compared using one-way anova sidak's test frequencies cases cardiac remodeling
Weight: 72.67 Paper Topics: presented cats follow cobalamin concentrations previously investigated cats little information available concerning hypocobalaminemic cats aimed assess frequency hypocobalaminemia large cohort gastrointestinal signs epidemiological clinical biological follow-up characteristics hypocobalaminemic cats1495 cats gastrointestinal signs cobalamin assay simultrac-snb radioassay kit vitaminb12 mpbiomedical performed 2007 2014 ldhvet laboratory retrospectively included study259 173% cats gastrointestinal signs hypocobalaminemia: majority domestic short hair 47% males 84% castrated 53% females 94% castrated aged months 18 years main clinical signs included chronic diarrhea 93% weight loss 71% polyuropolydypsia 48% vomiting 43% polyphagia 38% fatigability 36% dysorexia 19% median duration months diagnosis cobalamin values ranged 44
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ESVEXenotransfusion of canine blood to cats may be a life-saving procedure when treating an acute anaemic syndrome and compatible feline blood cannot be obtained. Published evidence in a limited number of cases dating from the 1960s indicates that cats do not appear to have naturally-occurring antibodies against canine red blood cell antigens. In fact compatibility tests before the first transfusion did not demonstrate evidence of agglutination or haemolysis of canine erythrocytes in feline serum and no severe acute adverse reactions have been reported in cats receiving a single transfusion with canine blood. Severe acute reactions not reported so far cannot however be excluded and we decided to perform a pilot study to evaluate the presence of naturally occurring antibodies against canine red blood cell antigens in cats and viceversa. Surplus material from diagnostic samples (blood EDTA and blood serum samples) of 13 cats and 24 dogs was used to perform test-tube major and minor cross-match tests (at 37°C, 4°C and room temperature (RT)) and blood typing, after obtaining the informed consent from owners. Hemolysis, macro-and micro-ag-glutination were investigated in each test tube and were considered markers of a positive matching. Blood from each cat was tested with blood from 2 to 6 different dogs for a total of 49 major and minor cross-matchings each one performed at the 3 different temperatures of incubation. Thirty-eight overall major cross-matchings proved positive at 37°C, 33 at RT and 39 at 4°C respectively. The minor cross-matching was positive in all but 2 tests performed at 37°C. No cat tested totally negative (37°C, 4°C, RT) at both major and minor cross-matching procedures performed towards any single dog. Ten cats experienced positive major and minor cross-matching at 37°C, RT and 4°C towards 1-3 different dogs. Five cats were positive in the major cross-match, at least at 37°C, towards 1-3 different different dogs. Seven cats obtained a positive major cross-match at RT and/or at 4°C towards 1-5 dogs. Only 2 cats tested completely negative at 37°C, RT and 4°C, in one out of the 4 different major cross-matchings performed. In conclusion, naturally occurring antibodies against canine red blood cell antigens appear to be frequently detected in cats as well as those against feline red blood cell antigens in dogs. Xenotrasfusion of canine blood to cats should only be performed after the selection of a compatible donor by means of at least a negative major cross-match test result.
ESVIM-O-4 DETECTION OF ANTI-ASPERGILLUS IMMUNOGLOBULINWe used liver microsomes from cats (16 individual and pooled), dogs (pooled), and humans (pooled). These liver samples were incubated at 37°C in a water bath with MPA and UDP-glucuronic acid or UDP-glucose. UDP-glucose was studied since MPA glucoside is a minor metabolite in humans but may be a major metabolite in other species. HPLC-MS was used to determine concentrations of MPA-glucuronide (phenol and acyl) and MPA-glucoside (phenol) formed by incubation.
ESCG-P-4 ASSESSMENT OF IMAGE QUALITY PRODUCED BYFive healthy Beagle dogs were included into this prospective study. All dogs were clinically examined, given a clinical score using the canine IBD activity index (CIBDAI) scoring system, also gastrointestinal endoscopy was performed. Mucosal biopsy specimens from duodenum were examined histologically from a board certified pathologist using the WSAVA grading. Biopsy incubation of 8-10 endoscopical mucosal biopsies in tissue culture medium with 3 H-labeled progesterone in the absence of any stimulation was performed. The mean age of the included dogs was 3.24+1.9 years, the mean weight was 17.8+1.8 kg. All Beagle dogs had a mean clinical score of 0+0. The mean WSAVA scoring was 2+1.2. After 4 hours, supernatant was harvested and radioactive progesterone metabolites formed were detected using high performance liquid chromatography plus liquid scintillation counting.
Five healthy Beagle dogs were included into this prospective study. All dogs were clinically examined, given a clinical score using the canine IBD activity index (CIBDAI) scoring system, also gastrointestinal endoscopy was performed. Mucosal biopsy specimens from duodenum were examined histologically from a board certified pathologist using the WSAVA grading. Biopsy incubation of 8-10 endoscopical mucosal biopsies in tissue culture medium with 3 H-labeled progesterone in the absence of any stimulation was performed. The mean age of the included dogs was 3.24 + 1.9 years, the mean weight was 17.8 + 1.8 kg. All Beagle dogs had a mean clinical score of 0 + 0. The mean WSAVA scoring was 2 + 1.2. After 4 hours, supernatant was harvested and radioactive progesterone metabolites formed were detected using high performance liquid chromatography plus liquid scintillation counting.
Weight: 72.34 Paper Topics: approximates purpose study investigate pacing modality vvi vvir vdd closely intrinsic sinus rate dogs degree av block 3avb range typical daily activities secondary aim study determine echocardiographic parameters different pacing modalities hypothesized vdd closely track intrinsic sinus rate activities echocardiographic dimensions different av asynchronous vvi vvir av synchronous vdd pacing modalitiesdogs prospectively recruited following implantation st jude dual-chamber pacemaker 3avb dogs evidence sinus node dysfunction clinically important arrhythmias 3avb excluded dogs programmed vdd study entry average lowest highest heart rates vdd pacing determined average heart rate programmed base rate vvi vvir highest heart rate programmed upper tracking rate vvir threshold
Weight: 63.76 Paper Topics: dogs idiopathic hyperlipidemia ih common condition miniature schnauzers ms 75% years old affected >45% dogs having moderate severe fasting hypertriglyceridemia htg combined hypercholesterolemia hchol severe htg poses increased risk development conditions eg pancreatitis insulin resistance recent studies humans suggest ih associated low-grade inflammation involved pathogenesis diseases associated ih risk complications decreases medical control ih given biomarkers inflammation investigated ms ih aims study evaluate serum calprotectin s100a12 concentrations i healthy ms ms ih ii ms ih response dietary intervention management ih serum samples collected 152 clinically healthy ms study questionnaire completed dog confirm health status medication history serum triglyceride
Weight: 61.45 Paper Topics: steroid purpose study demonstrate efficacy of suggest regimen for oral treatment csu patients refractory high dosage antihistamines conducted retrospective chart review patients diagnosed urticaria feburary 1 2012 december 31 2014 total 98 patients csu included these 16 patients 163% antihistamineresistant prescribed 2-week course steroid thirteen patients 812% successfully controlled antihistamines stopping course second course steroid induced remission additionally patients 125% adverse events complications associated oral steroid observed study period study demonstrated excellence 2-week course oral corticosteroid antihistamine-resistant csu propose standardized corticosteroid treatment regimenthe altered distribution follicular helper cells predict pronounced clinical course primary sjogren's syndrome margit zeher division
Weight: 56.76 Paper Topics: tests dneasy canine leishmaniasis important infectious disease worldwide commonly used antibody falsely negative direct detection pathogen polymerase chain reaction pcr necessary however pcr performed specialized laboratories available developed countries aim study evaluate sensitivity specificity rapid in-house pcr diagnosis canine leishmania infantum infection prospectively 515 samples 251 dogs 201 edta blood samples 244 conjunctival swabs 19 lymph node aspirates 51 bone marrow aspirates analysed presence leishmania dna using pcrun biogal galed labs acs ltd kibbutz galed israel results compared real time pcr gold standard identification leishmania kinetoplast dna minicircle dna extraction conducted using blood tissue kit qiagen gmbh sensitivity specificity
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ESVONC -European Society of Veterinary OncologyDisclosures: No disclosures to report. Adjunctive use of nutraceuticals to treat human cancers has shown promise, but little work has been done in canine neoplasia. Specifically, the multi-modal effects of polyphenols and carotenoids have been established in human cell lines and rodent models, with limited work in canine cells. We have previously identified two natural ingredients, turmeric extract (TE) and rosemary extract (RE), which worked synergistically to reduce neoplastic cell growth, and had no cytotoxic effect on normal dermal fibroblasts. This combination had an additive or synergistic effect when used with the chemotherapeutic agents toceranib or doxorubicin hydrochloride. The purpose of this in-vitro study was to examine mechanisms of action of this cocktail. Three canine neoplastic cell lines representing a variety of tumors were used: C2 (mastocytoma), CMT-12 (mammary gland carcinoma), and D17 (osteosarcoma). Apoptosis was determined using Annexin V staining and by a commercially available caspase-3/7 cleavage assay. Cell cycle changes using propidium iodide staining, generation of reactive oxygen species using Dihydrorhodamine123, modulation of cellular efflux pumps, and cellular accumulation of curcumin were analyzed by flow cytometry. Perturbation of various cell signaling pathways was assessed after 12 and 24 hours of incubation by western blotting. Cells were treated with 6.3 μg/mL of extract individually, a combination (3.1 μg/mL of each extract), or vehicle control. Comparisions between these four treatment groups were analyzed using a one-way ANOVA followed by Tukey's post-hoc analyses. The combination treatment induced apoptosis in all cell lines, beyond the effects of TE alone, after 36 hours of incubation. Both extracts had a significant antioxidant effect (P < 0.05). CMT-12 cells were the most susceptible to treatment (40% Annexin V positive; 5-fold increase in caspase cleavage). The presence of RE significantly increased the cellular accumulation of TE as indicated by an increase in fluorescence, with the CMT-12 cell line showing the greatest accumulation. Western blotting showed an increase in the amount of activated c-jun N-terminal kinase (JNK), although the differences varied across cell lines. TE and RE interact synergistically to induce apoptosis in-vitro; mechanisms may include RE increasing cellular accumulation of TE and activation of JNK.
8. ABSTRACTS C01 Pharmacodynamics of Entresto® (Sacubitril/Valsartan) Versus Placebo in Dogs with Preclinical Myxomatous Mitral Valve Disease C02 Trimethylamine-N-Oxide and its Precursors in Dogs with Degenerative Mitral Valve Disease Comparison Between the Effects of Torsemide and Furosemide on the Renin-Angiotensin-Aldosterone System of Normal Dogs C04 Transvenous Echocardiography in Conscious Sedated Horses C06 Complications and Outcomes of Multi-Institution Transvenous Pacemaker Implantation
Weight: 53.67 Paper Topics: 90cm structures objective assess diagnostic utility feasibility using 3-10mhz french intracardiac echocardiography ice catheter acuson acunav® evaluate cardiac function conscious horses sedated xylazineten apparently healthy horses 458-619kg texas a&m uni- procedure tolerated mild intermittent ventricular supraventricular arrhythmias resolved ice catheter repositioningwe conclude ice feasible safe allows acquisition repeatable diagnostic imaging planes conscious sedated horses potentially useful clinical situations tte technically difficult perform poorly tolerated horsesjulia r treseder nicole l leblanc katherine f scollan oregon state university corvallis or usa sotalol class iii anti-arrhythmic drug commonly prescribed treatment ventricular tachyarrhythmias dogs antiarrhythmic effects sotalol mediated increase action potential duration prolongation
Weight: 44.40 Paper Topics: passed cords bougie used 46 technique does use bronchoscopic control bougie tracheal tube tt trachea tt withdrawn cuff vocal cuff fully inflated tt advanced using bougie guide cuff impacts vocal cords gas- table complications type numbervisible cut bougie tt pulled far bougie murphy eye tt failure advance blue rhino bleeding late complication case seldinger needle blocked bougie material patient hard collar case seldinger needle perforated tt cuff tt prevented tracheostomy cuff sealing case seldinger wire passed murphy eye tt dilation process caused tt advance trachea second passage
Weight: 41.83 Paper Topics: plasma role introduction extracellular release atp important modulator immune response atp concentration increased sepsis 1 ifnγ plays critical host defense promoting th1 phenotype bacterial clearance low ifnγ levels associated th2 phenotype consistent critical illness anergy 2 reported 100 300 mm atp increased lps/pha-stimulated il-10 secretion human blood 3 higher il-10/ifnγ ratio shifts immune phenotype th1 th2 response studied effect atp lps-stimulated il-10 ifnγ secretion standardized ex-vivo human blood culture methods venous blood 10 healthy volunteers drawn tubes containing 10 ng lps/ml ilcso edi gmbh reutlingen germany incubated 100 mm atp respectively 37°c 24 hours supernates separated frozen